PCR

HiFi DNA polymerase is ideal for amplification of DNA fragments that require high-fidelity. High fidelity is achieved by an optimal blend of high performance YEAtaq DNA polymerase and Accu DNA polymerase, a Pyrococcus proofreading (3'→5' exonuclease activity ) enzyme. This formulation achieves greater yields with higher fidelity than standard DNA polymerases such as Taq. HiFi is a quality choice for sensitive applications. Because of the presence of Accu DNA polymerase in the blend, dUTP, dITP and primers containing these nucleotides should not be used in PCR because they hinder DNA synthesis. HiFi DNA polymerase is offered in two different packages, one with dNTPs mix and the other without. HiFi DNA Polymerase is also available in 2× ready-to-use premix format with a green dye.

YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. It is able to withstand repeated heating to 95°C without significant loss of activity. The enzyme catalyzes 5’→3’ synthesis of DNA, has no detectable proofreading 3’→5’ exonuclease activity and possesses low 5’→3’ exonuclease activity. It also exhibits deoxynucleotidyl transferase activity, which normally results in the addition of extra adenines at the 3’-end of PCR products, required for the DNA ligation to TA vector.

Yeastern Biotech offers YEAtaq DNA Polymerase in two different packages: one with dNTPs mix and the other without. In addition, a lyophilized version of this enzyme will be on the market later soon this year. 

The enzyme is in a recombinant form expressed in E. coli.