T&A™ Cloning Kit
Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech's T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.
The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3'-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.
- Fast ligation, completed in only 5 minutes
- High transformation efficiency
- More accurate results
- Accept a wide range of inserts with different sizes
- Two types of ligation buffers provided for your convenience
- Allow blue/white screening
- Contain ampicillin marker for antibiotic selection
- Include M13 primer sites for convenient sequencing