Total RNA kit (Cell Blood)
The Total RNA kit (Cell/Blood) is designed specifically for purifying total RNA from fresh whole human blood and cultured cells. Detergents and a chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound to the glass fiber matrix of the spin column and once any contaminants have been removed using the Wash Buffer, the purified total RNA is eluted by the Elution Buffer.
• Format : spin column (centrifuge)
• Yield : Up to 30 μg
107 x Culture Cells, 300 μl of blood
RT-PCR, Northern blotting, primer extension, mRNA selection cDNA synthesis