YEAtaq DNA Polymerase


YEAtaq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. It is able to withstand repeated heating to 95°C without significant loss of activity. The enzyme catalyzes 5’→3’ synthesis of DNA, has no detectable proofreading 3’→5’ exonuclease activity and possesses low 5’→3’ exonuclease activity. It also exhibits deoxynucleotidyl transferase activity, which normally results in the addition of extra adenines at the 3’-end of PCR products, required for the DNA ligation to TA vector.

Yeastern Biotech offers YEAtaq DNA Polymerase in two different packages: one with dNTPs mix and the other without. In addition, a lyophilized version of this enzyme will be on the market later soon this year. 

The enzyme is in a recombinant form expressed in E. coli.

Ref.: FYT001-500U


Features

  • Thermostable : half life is more than 40 min at 95°C.
  • 10× Reaction Buffer is supplied with two components: KCl and (NH4)2SO4, the latter allows for PCR at wide range of magnesium concentrations and decreases non-specific priming.
  • Incorporates modified nucleotides (e.g., dUTP, dITP, biotin-, digoxigenin-,fluorescently-labeled nucleotides).
  • The error rate of YEAtaq DNA Polymerase in PCR is 2×10-5errors per nucleotide per cycle.

 

Applications

  • Cloning
  • Screening
  • Primer extension
  • Terminal dA tailing
  • Routine PCR amplification of DNA fragments up to 3 Kb
  • DNA labeling
  • DNA sequencing

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72°C.

 

Quality Control

  • Nuclease activity is not detected after incubation of 1 μg λ /HindIII DNA with 5 units of YEAtaq DNA polymerase in 50 μl reaction buffer for 18 hours at 37 °C.
  • The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate tests. Functional test is performed by PCR.

Galmedic Suministros Médicos y de Laboratorio, S.L.

  Polígono de Pocomaco, Quinta Avenida, Nº 32 ,
A Coruña, 15190, (A Coruña)

  981142500

  981141410